HPLC Columns
Waters Corp Waters Atlantis HILIC OBD Prep Column, 100Å, 5 μm, 30 mm x 150 mm
Atlantis HILIC Silica columns are designed for use with high organic mobile phases (>80% acetonitrile) to retain analytes that are too polar for reverse-phase chromatography. The HILIC separation mechanisms offer orthogonal analyte selectivity compared to traditional chromatographic reversed-phase approaches.
- Optimum Bed Density (OBD) design ensures uniform density, void elimination, and resistance to mechanical bed failure.
- Ensures scalability from analytical to preparative chromatography by matching bed densities of equivalent analytical columns.
- Offers long, predictable lifetimes and exceptional resistance to mechanical bed failure.
- Enables fast, efficient lab-scale separations and direct scaling from UPLC, UHPLC, or HPLC to purification.
- Utilizes 100Å pore size and 5 μm particle size for preparative applications.
Waters Corp Waters Prep Column, BEH C18, 5 μm, 50 × 50 mm
This preparative column features robust BEH C18 sorbent for HPLC method development, compatible with a wide mobile phase pH range of 1–12. It offers excellent low pH stability and minimal column bleed for various HPLC separations.
- Chemistry: C18
- Separation mode: Reversed phase
- Particle substrate: Hybrid
- pH range: 1–12
- Temp limits: 80°C (low pH), 60°C (high pH)
- Max pressure: 6000 psi (415 bar)
- Endcapped; Low silanol activity
- Particle: Spherical, 5 μm
- Pore size: 130 Å
- Format: OBD prep column
- Surface area: 185 m²/g
- System: HPLC preparative
- Technique: LC/MS, LC
- USP classification: L1
- Dimensions: 50 mm ID x 50 mm length
- Carbon load: 18%
Waters Corp Waters XBridge BEH C18 OBD Prep Column, 130Å, 5 μm, 19 mm X 250 mm
These columns are designed for ruggedness and longevity in LC methods, allowing chromatographers to use mobile phases in a wide pH range. Built with Optimum Bed Density (OBD) technology, they boost productivity with higher recoveries and longer column lifetimes, enabling fast, efficient, lab-scale separations and direct scalability from UPLC, HPLC, and UHPLC screening to lab-scale purification.
- C18 sorbent for wide mobile phase pH (1 to 12).
- Trifunctionally bonded C18 ligand for universal HPLC separations.
- Superior low pH stability and ultra-low column bleed.
- High pH and temperature stability for increased basic compound loading.
- OBD technology provides higher recoveries, extended column lifetimes.
- Direct scalability from UPLC, HPLC, and UHPLC to lab-scale purification.
- Resists mechanical bed failure, ensures consistent performance and reduced costs.
Waters Corp Spherisorb Column, 80Å, 5 μm, 4.6 mm x 100 mm
Spherisorb SAX columns contain a silica-based quaternary ammonium bonded sorbent. The column is listed as USP L14 and is used for anion-exchange separations.
- Chemistry: Anion-exchange
- Separation mode: Ion-exchange
- Particle substrate: Silica
- pH range: 2 to 8
- Maximum pressure: 6000 psi (415 Bar)
- Endcapped: No
- Bonding technology: SAX
- Silanol activity: High
- Particle shape: Spherical
- Endfitting type: Parker-style
- Format: Column
- Surface area: 220
- System: HPLC
- USP classification: L14
- Inner diameter: 4.6 mm
- Length: 100 mm
- Carbon load: 4%
- Pore size: 80 Å
- Particle size: 5 μm
- Units per package: 1
Waters Corp Spherisorb SCX Column, 80Å, 10 μm, 4.6 mm x 150 mm
Spherisorb SCX columns contain an anionic silica-based sorbent. This strong cation-exchange sorbent is used to separate positively charged ionic species.
- Cation-exchange chemistry
- Ion-exchange separation mode
- Silica particle substrate
- pH range of 2 to 8
- Maximum pressure of 6000 psi (415 bar)
- Not endcapped
- SCX bonding technology
- High silanol activity
- Spherical particle shape
- 10 μm particle size
- Parker-style endfitting type
- 80 Å pore size
- Column format
- 220 surface area
- HPLC system
- 4.6 mm inner diameter
- 150 mm length
- 4% carbon load
- 1 unit per package
Waters Corporation Column, C18, 300Å, 3.5 μm, 4.6 mm X 50 mm
The XBridge BEH300 C18 Peptide Separation Technology (PST) columns are optimized and QC tested for peptide separations. They feature a wide-pore (300Å) trifunctionally bonded BEH particle, offering the widest usable pH range, superior low pH stability, and ultra-low column bleed. These columns are designed for assaying samples in proteomics, protein characterization, and peptide synthesis.
- Optimized and QC tested for peptide separations
- Wide-pore (300Å) trifunctionally bonded BEH particle
- Widest usable pH range
- Superior low pH stability
- Ultra-low column bleed
Waters Corp XBridge Peptide BEH C18 Column, 130Å, 10 µm, 4.6 mm X 100 mm, 1/pk
The XBridge BEH130 C18 Peptide Separation Technology columns are optimized and QC tested for peptide separations. The small-pore (130Å) trifunctionally bonded BEH particle offers you the widest usable pH range, superior low pH stability, and ultra-low column bleed to assay samples for proteomics, protein characterization, and peptide synthesis.
Imtakt PRESTO FF-C18 HPLC COLUMN
This is the world’s first 2u non-porous column. This extremely efficient material works very well for separation of large molecules like polymers, surfactants, protein and even DNA. It has a wide effective analysis range from 5kDa to 50 MDa. 150 x 4.6 mm column
Waters Corp XBridge BEH C18 XP Column, 130Å, 2.5 µm, 4.6 mm X 30 mm, 1/pk
As a workhorse for HPLC method development, XBridge BEH C18 sorbent can be used over an entire range of mobile phase pH (1-12). The trifunctionally bonded C18 ligand provides you with a universal column for most HPLC separations and gives you the widest usable pH range, superior low pH stability, and ultra-low column bleed. Packed in ultra-low dispersion hardware, 2.5 µm eXtended Performance [XP] columns enable exceptional separation performance, robustness and throughput for HPLC assays while enabling a seamless transition path toward UPLC adoption.
Waters Corp XBridge Column, BEH Phenyl, 2.5 μm, 4.6 x 30 mm
These XBridge BEH Phenyl columns offer complementary selectivity to C18 stationary phases, ideal for polyaromatic compounds. They are chemically stable phenyl sorbents, providing ultra-low column bleed, extended lifetimes, and excellent peak shape. Packed in ultra-low dispersion hardware, the 2.5 μm XP columns deliver exceptional separation performance and robustness for HPLC assays, facilitating UPLC adoption.
- Unparalleled column longevity for chromatographic analysis.
- Wide operating range for mobile phase pH (1 to 12) and temperature.
- Facilitates seamless UPLC technology conversion.
- Provides alternative selectivity for analytes with aromatic rings.
- BEH particle technology ensures robust technique development and pH stability.
- Withstands a maximum pressure of 10,000 psi (690 Bar).
Waters Corp Waters Column, BEH HILIC, 2.5 μm, 4.6 mm × 30 mm, 130 Å
These BEH HILIC columns utilize an unbonded BEH substrate, designed for retaining and separating highly polar compounds. They provide efficient, reproducible HPLC HILIC separations with greater ruggedness and extended lifetimes due to BEH particle chemical stability. The 2.5 μm eXtended Performance (XP) columns, packed in ultra-low dispersion hardware, offer exceptional separation performance and throughput for HPLC, enabling smooth UPLC transition.
- Retains and separates very polar compounds.
- Optimized for efficient, reproducible HPLC HILIC separations.
- Rugged and long-lasting due to BEH particle chemical stability.
- Packed in ultra-low dispersion hardware for 2.5 μm XP.
- Exceptional separation performance, robustness, and throughput for HPLC.
- Seamless transition path toward UPLC adoption.
Waters Corp Column, PFP/Fluoro-Phenyl, 2.5 μm, 4.6 mm, 50 mm
This column is designed to maximize selectivity differences, offering maximum method development freedom, particularly when using low pH mobile phases. Its non-encapped sorbent provides enhanced retention of acidic compounds. The 2.5 μm eXtended Performance [XP] columns are packed in ultra-low dispersion hardware, enabling exceptional separation performance, robustness, and throughput for HPLC assays, while also providing a seamless transition path toward UPLC adoption.
- Designed to maximize selectivity differences, providing maximum method development freedom, especially with low pH mobile phases.
- Non-encapped sorbent enhances retention of acidic compounds.
- Packed in ultra-low dispersion hardware.
- 2.5 μm eXtended performance [XP] columns enable exceptional separation performance, robustness, and throughput for HPLC assays.
- Enables a seamless transition path toward UPLC adoption.
Waters Corp Column, Phenyl-Hexyl, 2.5 μm, 4.6 mm × 75 mm
These XSelect CSH Phenyl-Hexyl columns provide alternative analyte selectivity, featuring a robust, low bleed C6 phenyl-ligand that selectively retains polyaromatic compounds for method development.
- Alternative analyte selectivity for method development.
- Robust, low bleed C6 phenyl-ligand for pi-pi interactions.
- Ultra-low dispersion hardware; 2.5 μm XP columns for exceptional HPLC separation, robustness, and throughput.
- Fully compatible with HPLC, UPLC, and UHPLC technologies.
- Improved performance, backpressure, loading capacity, and particle size scalability over core-shell and fully porous particle columns.
- Charged Surface Hybrid (CSH) technology enhances LC performance in acidic, low ionic strength.
- Increases selectivity differences and enhances acidic compound retention (non-end-capped sorbent).
Waters Corp Column, CSH Phenyl-Hexyl, 130Å, 2.5 μm, 4.6 x 30 mm
This column is a CSH Phenyl-Hexyl XP type, with specifications of 130Å, 2.5 μm, 4.6 mm X 30 mm, and comes as 1 package.
- Product type: (U)HPLC analytical column.
- Chemistry: Phenyl-Hexyl (CSH Phenyl-Hexyl XP).
- USP class: L11.
- Carbon load: 14.0%.
- Bet surface area: 185.0 m2/g.
- Hardware material: Stainless steel.
- pH range: 1.0 (minimum) to 11.0 (maximum).
- Temperature range: 45°C to 80°C.
- Mode of separation: Reversed phase Chromatography (RP).
- Technology: U(H)PLC.
- Matrix: Hybrid.
- Particle morphology: Spherical, fully porous.
- Pressure stability: 1240 bar.